Metrohm Inline Dialysis Manual do Utilizador descarregar pdf (Página 24)

4.1 Operation

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IC equipment: Dialysis

Measuring the standard solution with dialysis

￭ Set the dialysis time in the software to 5 minutes.

￭ Immerse the sample aspiration capillary in the standard solution

used in step 1.

￭ Start a determination in the software and wait until the chroma-

togram has been evaluated.

Additional measurements

￭ Increase the dialysis time in the software by 1 minute each time

and start the determination.

￭ Repeat the measurements until the measured values are stable.

Determining the optimum dialysis time

￭ The recovery rates can be calculated from the result without dialy-

sis and the results with dialysis. You can determine the optimum

dialysis time by recording the recovery rate in relation to the dialy-

sis time.

4.1.2 Recommended procedure for the dialysis

For sample determination with dialysis, it is best to proceed according to

the following sequence:

Carrying out a dialysis

Preparing the acceptor solution

￭ To avoid disruptions caused by air bubbles in the acceptor flow,

degas the ultrapure water used as acceptor solution for at least

10 minutes.

Preparing the sample

￭ To avoid blockage of the sample channel, always centrifuge sam-

ples with a high content of suspended or solid particles in a tab-

letop centrifuge for 5 minutes at 10,000 rpm.

Putting the IC system into operation

￭ Switch on all required devices.

￭ Start the software and load the required IC system.

￭ Condition the IC system.

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Metrohm Inline Dialysis Manual do Utilizador Página 24